We aim to develop a next-generation enzymatic DNA synthesis technology that enables rapid, low-cost, and sustainable production of complex nucleic acids. This platform will empower synthetic biology, drug discovery, and vaccine development.

We have developed a novel engineered enzyme capable of template-independent DNA synthesis that can incorporate reversibly blocked nucleotides. Initial validation experiments confirmed its activity. We are optimising reaction conditions to further improve synthesis efficiency. Our goal is to demonstrate the enzymatic synthesis of a small gene using blocked nucleotides and further engineer the enzyme to enhance performance.

The project started at the end of Paola’s PhD on polymerase engineering for xenobiotic nucleic acid (XNA) synthesis, when she explored the idea of engineering a novel polymerase for de novo DNA, RNA, and XNA synthesis. Early experiments showed promising results. During her postdoctoral research at ETH Zurich, she met Jelmi and Jan-Phillip, and together they decided to further develop the project and explore its potential.